BSRT Graduate School

Courses

Science Communication - Advanced Scientific Drawing

4/9
Description:

 

Take a look at benchmarks in Science like Cell- and Nature- Press and realize how much work is dedicated into the visual aspects of high quality publication. In this 2-hour practical course, we will explore and discuss the world of scientific drawing. Visualization of your scientific ideas, biological processes, workflows and data is today a central aspect in scientific communication. May it be your next paper, review, your thesis or your next grant application, all will benefit from thoughtful and aesthetic graphics that facilitate your ideas and work.

 

We will discuss the basics of scientific drawing and:

  • Compare different graphic software for the creation of individual artwork including CorelDraw, InkScape, PhotoShop and cloud-based solutions such as Biorender
  • Discuss at the example of good and bad artwork which artistic decisions place a scientific message into focus
  • Each participant will use his/her PhD project idea to create an own scientific drawing of high quality that will be presented, discussed and improved over the course

 

The course is planned to be held in person. If the number of participants is too low, it will be held online.

 

 


Materials - Macromolecular Chemistry

0/-
Description:


After a short repititon of basic mechanisms in chemistry and intra- and intermolecular interactions, the methods of macromolecular chemistry: synthesis, characterization and processing will be introduced. Modern materials, new methods and their application in the biomedical field and following prerequisites for the materials are discussed. Hands on macromolecular synthesis: a polymer will be synthesized and characterized.

 

Course Materials and Techniques: A handout for all participants is available
 

 

For questions and registration please contact Michael Schroeter (michael.schroeter@hereon.de, Cc bianca.kuehn@charite.de)

 


Materials - Polymer Chemistry of Biomaterials

0/-
Description:

 

Polymer based biomaterials, actively moving polymers and multifuctional polymers for biomedical applications will be introduced. Biodegradable polymers, hydrogels and biomimetic polymer materials are introduced, and applications by way of example explained. In the practical part a hydrogel will be synthesized and characterized to demonstrate the influence of synthetic parameters on the macroscopic properties of the material.

 

Course Materials and Techniques: A handout for all participants is available

 

 

For questions and registration please contact Michael Schroeter (michael.schroeter@hereon.de, Cc bianca.kuehn@charite.de)

 

 


Peer to Peer - 3R - REFINE - REDUCE - REPLACE - meets Design Thinking

0/-
Description:

The 3R principle (Replacement, Reduction and Refinement of Animal Experiments) is of utmost significance for research with animals and offers great potential for advancement. Design Thinking is a method to facilitate problem-solving and the development of innovative solutions in multi-disciplinary teams.

 

In this workshop we work in teams on the ethically and scientifically relevant challenge: “Accelerating the implementation of the 3Rs in animal research”. The workshop is supported by short briefings about basics concerning the 3Rs and animal research. The teams will then use design thinking methods to explore the complexity of the challenge, get to know different perspectives and develop a common understanding. All gathered information, perspectives and experiences are incorporated into the solution process creating a space between user needs and feasibility. The teams create tangible solutions by prototyping and testing of selected ideas.

 

 

For registration please contact bianca.kuehn@charite.de

 

 


Preclinical Studies - Animal experiments in science

3/12
Description:

 

A remote course!

 

Program - Day 1 (9 am to 1 pm)

Geetings - Agenda

A short history of animal experimental work

Ethical aspects

Putting animal experimental work into prospective

How to write the ethical statement for an animal proposal

Practical approach: an ethical statement

Ethics and moral –discussion

 

Program - Day 2 (9 am to 5 pm)

The 3 Rs Legalities

The different parts of an animal proposal

Who can apply for an animal experiment?

The dreaded statistics

Severity assessment

Humane end points

The score sheet

NTP

Documenting an animal experiment

 

Learning Objectives

1) Animal experiments and ethics

2) Get acquainted with the law

3) The 3 R rule

4) severity assessment

5) learn what it entails to write an animal proposal

6) Statistical planning – an experience

 

 


Skeletal Development

2/2
Description:

This practical course gives an introduction into the methods and technology used to investigate skeletal and muscle development in the mouse. The skeleton evolves from mesenchymal cells which differentiate into bone forming osteoblasts and cartilage forming chondrocytes. Through a complex genetic program these cells eventually form the mature bone, keep this tissue in homeostasis and provide a cellular resource for healing. Muscles on the other hand form from progenitors arising in the somites, which reach their final destionations via a migration process. During muscle formation a subpopulation of myogenic cells is arrested in the undifferentiatied state and serves as stem cells (so-called satellite cells) for regenerative processes in the adult. In this course, the participants will be able to investigate gene and protein expression during skeletogenesis and myogenesis in the mouse and to analyze skeletal malformations in mouse models for human skeletal disease. This course is aimed at providing a basic understanding for the genetic mechanisms that govern musculoskeletal patterning and differentiation during embryogenesis and to provide knowledge on how to analyze musculoskeletal phenotypes in animal models.

 

Course Materials and Techniques

The onset of skeletal and myogenic development in the embryo will be analyzed by RNA in-situ hybridisation, the formation of muscles and muscle stem cells will be analized by immunostaining on tissue sections. The differentiation of cartilage and bone will be analyzed by histology of embryonic tissue sections. The patterning and morphogenesis of the skeleton will be analyzed in wild type and mutant mice by whole mount skeletal staining.


Skeletal Development - Introduction to mouse bone analysis

1/4
Description:

 

This course is designed for students working on mouse bone phenotyping. The course introduces to the participants the basics of bone development and techniques to investigate skeletal morphological abnormalities found in mutant mice, using osteopetrosis and osteoporosis as examples. Each lecture is followed by a practical session in which participants will work on histomorphometric analysis and microCT analysis of mouse bone specimens.

 

 


Mechanics - Basic theory and measurement concepts

0/10
Description:

In this course the basic tools for the description and measurement of mechanical material behaviour will be presented.

 


Part A, day 1+2:
- Classification of materials (crystalline, amorphous, compounds)
- Intrinsic electromagnetic interactions
- Introduction of a constitutive law
- Stress-strain curves
- Elastic Theory: Hooke's law, Isotropy, Anisotropy, Technical vs. Engineering Constants
- Plastic Deformation: Toughness, Strength
- Viscoelasticity

 

Part B (seminar), day 3:
- Students will solve basic mechanical problems related to research in regenerative therapies

 

Course Material and Techniques:
Ashby and Jones, 2005, Engineering Materials volumes 1 and 2, 3rd Ed.

L.D. Landau and E.M. Lifschitz, Theory of Elasticity, Butteworth-Heinemann, 1986. ISBN: 978-0750626330

T.H. Courtney, Mechanical behavior of materials, McGraw-Hill, Singapore 1990.

W.P. Mason, Physical Acoustics and the Properties of Solids, D.Van Nostrand Company, New York, 1958.


In Vivo Technology - Transgenic animal technology (rodents)

5/10
Description:

 

This course will give an introduction in the technologies used to generate and phenotype transgenic and knockout rodents.

 

Practical part I: Generation of genetically engineered animals (Elena Popova)

Practical part II: Phenotyping of transgenic rats

 

 


Histology

14/14
Description:

This theoretical and practical basic histological course is designed for students from all scientific fields with no or just a minimal experience and background in histological analyses. The course will give an overview on the histology of tissue with a special focus on musculoskeletal tissue and its regeneration. Evaluation of tissue processes can be achieved by conventional stainings as well as cell interactions with immunohistological stainings. In lectures, we will teach basic knowledge about tissue anatomy, tissue preparation, different common staining methods as well as the scientific benefit and application possibilities of histological analyses. In the accompanying practical part, we will apply the theoretical knowledge on tissue samples including the tissue embedding process, the preparation of plastic and paraffin sections, different conventional and immunohistological staining procedures, microscopy and histomorphometry. An insight into nanoindentation and confocal microscopy will also be included in the course.

 

Course Materials and Techniques: different tissue samples, tissue handling for histology, basic histological preparation and staining methods, microtome, microscope, histomorphometry, nanoindentation, confocal microscopy
 


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