BSRT Graduate School

Histology

TitleTimeTeacherRoom
Histology 02.11.2021 09:00 - 16:00 (Tue)Franka Klatte, Christian Bucher
Histology03.11.2021 09:00 - 16:00 (Wed)Franka Klatte, Christian Bucher
Histology 04.11.2021 09:00 - 13:00 (Thu)Franka Klatte, Christian Bucher
Title of Event: 
Histological characterization of tissues and cells
Optional or Mandatory: 
This course is optional for doctoral researchers at the BSRT
booked/available (including waiting list): 
8/8
maximum number of participants: 
8
Description:

This theoretical and practical basic histological course is designed for students from all scientific fields with no or just a minimal experience and background in histological analyses. The course will give an overview on the histology of tissue with a special focus on musculoskeletal tissue and its regeneration. Evaluation of tissue processes can be achieved by conventional stainings as well as cell interactions with immunohistological stainings. In lectures, we will teach basic knowledge about tissue anatomy, tissue preparation, different common staining methods as well as the scientific benefit and application possibilities of histological analyses. In the accompanying practical part, we will apply the theoretical knowledge on tissue samples including the tissue embedding process, the preparation of plastic and paraffin sections, different conventional and immunohistological staining procedures, microscopy and histomorphometry. An insight into nanoindentation and confocal microscopy will also be included in the course.

 

Course Materials and Techniques: different tissue samples, tissue handling for histology, basic histological preparation and staining methods, microtome, microscope, histomorphometry, nanoindentation, confocal microscopy
 

 

 

Day 1  (2/11/2021)   

9.00-10.00

Lecture: Tissue preparation and classical staining methods of connective tissues

Seminar Room A (0.0043); Franka Klatte-Schulz 

10.00-10.15

Break

10.15-16.00

Practical course: Preparation of plastic, cryo and paraffin sections

PMMA: Mineralized bone

Cryo: Soft tissue

Paraffin: Decalcified bone / Soft tissue

 

Group 1

Group 2

10.15-11.45

PMMA samples

Room: 1.0177

Sabine Stumpp, Marzena Princ

Cryo samples (2 persons)

Room: 1.0166 Gabriela Korus

Paraffin samples (2 persons)

Room: 1.0175 Aysha Schmock

11.45-12.45

Lunch break

12.45-14.15

Cryo samples (2 persons)

Room: 1.0166 Gabriela Korus

PMMA samples

Room: 1.0177

Sabine Stumpp, Marzena Princ

Paraffin samples (2 persons)

Room: 1.0175 Aysha Schmock

14.15-14.30

Break

14.30-16.00

 

Classic histological staining

Room: 1.0166

Sabine Stumpp, Marzena Princ

Introduction into µCT

Room: 01.0017

Mario Thiele

 

 

 

Day 2 (3/11/21)

 

9.00-10.00

Lecture: Specialized staining methods and visualization

Seminar Room A (0.0043); Christian Bucher

10.00-16.00

Practical demonstrations

Group 1

Group 2

10.00-11.30

Introduction into µCT

Room: 01.0017

Mario Thiele

10.00-11.30

Classic histological staining

Room: 1.0166

Sabine Stumpp, Marzena Princ

11.30-12.15

Lunch break

12.15-14.00

Immunohistochemistry

Room: 1.0166

Gabriela Korus

 

12.15-13.30

Introduction in histomorphometry

Room: 1.0015

Mario Thiele

13.30-14.00

Introduction into Nanoindentation

Room: 1.0177

Dag Wulsten

14.00-14.15

Break

14.15-15.30

Introduction in histomorphometry

Room: 1.0015

Mario Thiele

14.14-16.00

Immunohistochemistry

Room: 1.0166

Gabriela Korus

 

15.30-16.00

Introduction into Nanoindentation

Room: 1.0177

Dag Wulsten

 

 

Day 3 (4/11/21)

  

9.00-11.30

Practical demonstrations

Group 1

Group 2

9.00-10.15

 

Microscopy and Histo Quiz

Seminar Room C (1.0028)

Franka Klatte-Schulz

9.00-9.15

Introduction into confocal microscopy/Epifluorescence

Seminar Room B (0.0028)

Aaron Herrera, Christian Bucher

9.15-10.15

Confocal microscopy

Room: 1.0169

Aaron Herrera

Epifluorescence

Room: 1.0003

Mario Thiele

10.15-10.30

Introduction into confocal microscopy/Epifluorescence

Seminar Room B (0.0028)

Aaron Herrera, Christian Bucher

10.15-11.30

 

Microscopy and Histo Quiz

Seminar Room C (1.0028)

Franka Klatte-Schulz

10.30-11.30

Confocal microscopy

Room: 1.0169

Aaron Herrera

Epifluorescence

Room: 1.0003

Mario Thiele

11.30-13.00

Lunch and Feedback

Foyer

Franka Klatte-Schulz, Christian Bucher

 

 

 

 

 

 

 

 

 

 

 

 


Credits: 
1.5