BSRT Graduate School

Claudia Schlundt

claudia.schlundt@charite.de's picture
Nationality: 
Germany
Organisation: 
Charité - Universitätsmedizin Berlin
Institute: 
Julius Wolff Institute
Address: 

Augustenburger Platz 1 13353 Berlin

Campus: 
Phone: 
+49 (0)30 450559204
Fax: 
+49 (0)30 450559969
Track: 
Biology Track
At BSRT since: 
06.2013
Graduation: 
18.07.2017
PhD Supervisor 1: 
Georg Duda
PhD Supervisor 2: 
Hans-Dieter Volk
PhD Mentor: 
Katharina Schmidt-Bleek
Title of PhD project: 
Analyses of distinct immune cell subsets in the initial fracture hematoma in a mouse osteotomy model
CV: 

Since 09/2012 PhD Student at Julius Wolff Institute, Charité - Universitätsmedizin Berlin, Germany. 07/2011 to 02/2012 Scientific Researcher in the lab of Dr. Herling, Medical Department I at Universität zu Köln, Germany. 10/2009 to 06/2010 Diploma thesis in the group of Dr. habil. Baumgrass at Deutsches Rheuma-Forschungszentrum, Berlin, Germany. 09/2006 to 01/2007 ERASMUS Semester at Université Bordeaux I, France. 10/2004 to 10/2010 Diploma in Biology at Freie Universität Berlin.

Project Abstract: 

The bone and the immune system are interdependent on each other. Bone homeostasis is regulated by a well-defined interplay between osteoblasts and osteoclasts. Bone fracture healing starts with the formation of a hematoma due to the disruption of the blood vessels in the fractured area. The hematoma formation is accompanied by an inflammatory reaction. This early fracture healing phase is an indispensable step for the initiation und regulation of the regenerative healing process. T cells infiltrating the inflamed tissue play a key role in regulating this repair process by determining the local cytokine milieu. Recently, we could show that a specific depletion of CD8+ T cells improves the healing outcome. Following this strategy, a reduction of the pro-inflammatory reaction triggered by an immune cell subpopulation could be a promising approach to positively modulate the healing process. One part of my PhD project focusses on the analysis of the impact of regulatory T cells, a subpopulation of CD4+ T cells, on the bone fracture healing process as well as outcome. This will be realized in our already well-established mouse osteotomy model. After adoptive transfer of freshly isolated murine Tregs, the healing outcome will be analyzed after 21 days in comparison to an untreated wild type group. In addition, I will evaluate the healing process over 21 days in wild type mice. This evaluation includes the analysis of the spatial and temporal distribution of distinct immune cell subsets in the fractured bone as well as systemically. This will be done by histology and immunohistology as well as flow cytometry. The aim is to get a better and deeper understanding of the cell-cell and tissue-cell distribution and interaction of immune and bone cells during a normal healing situation and to determine positive effects of specific immune cell subsets on the regeneration process.

Presentations: 
Talk
Schlundt C, Reinke S, Geissler S, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Bone fracture healing outcome is dependent on the frequency of CD8+ T cells. 4th BSRT PhD Symposium, Berlin, 4-6 December 2013

Schlundt C, Wendler S, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Inflammation and angiogenesis are interdependent systems in tissue regeneration. World Conference on Regenerative Medicine, Leipzig, 23-25 October 2013

 
Schlund C, Schmidt-Bleek K, Könnecke I, Schell H, Volk HD, Duda GN. Immune cells and fracture healing. 3rd BSRT PhD Symposium, Berlin, 5-7 December 2012
Poster
Schlundt C, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Regulatorische T-Zellen: Positive Regulatoren in der Frakturheilung? Erste Ergebnisse. Deutscher Kongress für Orthopädie und Unfallchirurgie, Berlin, 20-23 October 2015
 
Schlundt C, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Interplay between the adaptive immune and bone system in fracture healing: Friend or foe? Annual Meeting of the American Society for Bone and Mineral Research, Seattle, UAS, 9-12 October 2015
 
Schlundt C, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Impact of the adaptive immune system in bone fracture healing. International Symposium on Bone Regeneration, Berlin, 11-13 June 2015
 
Schlundt C, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Regulatory T cells: The good guys in fracture healing? Preliminary findings. 5th BSRT PhD Symposium, Berlin, 3-5 December 2014
 
Schlundt C, Reinke S, Geissler S, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Frequency of CD8+ T cells determines bone fracture healing outcome. 5th International Conference on Osteoimmunology: Interactions of the Immune and Skeletal Systems, Kos, Greece, 15-20 June 2014
Schlundt C, Schell H, Serra A, Radbruch A, Volk HD, Duda GN, Schmidt-Bleek K. B cells regulate bone formation during fracture healing. Annual Meeting of the Orthopaedic Research Society, New Orleans, USA, 15-18 March 2014

Schlundt C, Duda GN, Könnecke I, Schell H, Volk HD, Schmidt-Bleek K. Immune cells kiss bone cells in fracture healing. 3rd Advanced Summer School: Interrogations at the Biointerface - Inflammation and Repair Interface, Porto, Portugal, 26-28 June 2013

Awards: 
Poster prize
Schlundt C, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Regulatory T cells: The good guys in fracture healing? Preliminary findings. 5th BSRT PhD Symposium, Berlin, 3-5 December 2014
 
Schlundt C, Schell H, Volk HD, Duda GN, Schmidt-Bleek K. Impact of the adaptive immune system in bone fracture healing. International Symposium on Bone Regeneration, Berlin, 11-13 June 2015
Thesis (BSc, MSc): 
Diploma Thesis: The effect of selected transcription factors on the signal transduction in T-cells.