BSRT Graduate School

Michael Sittinger

michael.sittinger@charite.de's picture
Organisation: 
Charité - Universitätsmedizin Berlin
Institute: 
Berlin-Brandenburg Center for Regenerative Therapies
Address: 

Charité Campus Virchow-Klinikum

Augustenburger Platz 1

13353 Berlin

Research Interests: 

The mission of our group is to develop core technologies based on multidisciplinary knowledge for cell biology, cell culture and biocompatible delivery materials. We primarily work with cells from mesenchymal tissues and have extensive experience in 3D cell cultures and in the translation of research into therapies. Our group has developed 3D cell and tissue culture systems, which comprise the use of mesenchymal cells and resorbable biomaterials in combination with temporary matrices to allow the formation of cartilaginous and osseous tissue in vitro and in animal models. Together with clinical and industrial partners, these techniques were translated from the bench to the bedside and those clinical therapies for orthopedic and oral surgery are now considered of the pioneering tissue engineering products on the European market.Mesenchymal stem cells (MSC) have the capacity for extensive self-renewal and to develop into distinct mesenchymal tissues. We hypothesize that therapy approaches using chemoattractant molecules and differentiation factors will allow the in situ recruitment of human MSC to sites of degenerated cartilage and their subsequent utilization for the directed regeneration of cartilaginous joint structures. Therefore, we characterize these cells using Fluorescence Activated Cell Sorting (FACS), real time-PCR (Polymerase Chain Reaction), differentiation assays and gene expression profiling via Affymetrix oligonucleotide arrays. The research group is participating in the European integrated project STEPS for the molecular analyses of connective tissues. Applied research activities of the group are increasingly based on regional collaborations with small biotech companies as well as Charité spin off companies. The Tissue Engineering Laboratory is integrated in the Berlin-Brandenburg Center of Regenerative Medicine with focus on translational technologies to translate results from discovery research into clinical application and product development as well as research data into regenerative therapies.